The magnetic nanoparticle-assisted isolation of cells, proteins, DNA, and other analytes from such complex matrices as whole blood, blood serum or plasma, urine, tissue extracts, food, and liquor are a powerful tool in biosensing, facilitating increases in analytical sensitivity and specificity. Magnetic nanoparticles find application in numerous fields of biomedicine, including drug delivery, tissue engineering, bioimaging, biosensing, and many others. Finally, we compared the colloidal stability at various pH values and ionic strengths and in relevant complex media (i.e., blood serum, plasma, milk, juice, beer, and red wine), as well as the heat stability, resistance to proteolytic digestion, and shelf-life of protein-coated nanoclusters. Fourth, the protein coronas of nanoclusters were studied using SDS-PAGE and Bradford protein assay. Third, we demonstrated the applicability of the obtained nanoclusters in biosensing by the development of a nuclear-magnetic-resonance-based immunoassay for the quantification of antibodies against tetanus toxoid. Second, we showed that protein coating does not significantly influence the r2 relaxivity of clustered nanoparticles however, the uniform distribution of individual nanoparticles inside the protein coating facilitates increased relaxivity.

First, we examined the influence of environmental parameters on the size of prepared nanoclusters and synthesized protein-coated nanoclusters with a tunable size.

In this work, we functionalized the nanoclusters of carbon-coated iron nanoparticles with four proteins: bovine serum albumin, casein, and gelatins A and B, and we conducted a comprehensive comparative study of their properties essential to applications in biosensing.

Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and transverse relaxivity (r2) of magnetic nanoparticles. The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine.

System shock ricardo milos